Visualization and Quantification of TGFβ/BMP/SMAD Signaling under Different Fluid Shear Stress Conditions using Proximity-Ligation-AssayPaul-Lennard Mendez 1,2, Leon Obendorf 1, Petra Knaus 1
1Institute for Chemistry and Biochemistry, Freie Universität Berlin, 2International Max-Planck Research School for Biology and Computation
Here, we establish a protocol to simultaneously visualize and analyze multiple SMAD complexes using proximity ligation assay (PLA) in endothelial cells exposed to pathological and physiological fluid shear stress conditions.