Results: Effect of Shear Stress on the SMAD Protein Interactions
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Conclusion
Transcript
This protocol enables scientists to quantitatively compare the number of transcription factor complexes under different shear stress conditions, and will be beneficial for research in vascular biology. The main advantage of this protocol is the us
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Here, we establish a protocol to simultaneously visualize and analyze multiple SMAD complexes using proximity ligation assay (PLA) in endothelial cells exposed to pathological and physiological fluid shear stress conditions.