Fibroblast Derived Human Engineered Connective Tissue for Screening ApplicationsGabriela L. Santos 1,2, Tim Meyer 1,2, Malte Tiburcy 1,2, Alisa DeGrave 1,2, Wolfram-Hubertus Zimmermann 1,2,3,4,5, Susanne Lutz 1,2
1Institute of Pharmacology and Toxicology, University Medical Center Goettingen, 2DZHK (German Center for Cardiovascular Research) partner site, Goettingen, 3Cluster of Excellence “Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells” (MBExC), University of Goettingen, 4Center for Neurodegenerative Diseases (DZNE), 5Fraunhofer Institute for Translational Medicine and Pharmacology (ITMP)
Presented here is a protocol to generate engineered connective tissues for a parallel culture of 48 tissues in a multi-well plate with double poles, suitable for mechanistic studies, disease modeling, and screening applications. The protocol is compatible with fibroblasts from different organs and species and is exemplified here with human primary cardiac fibroblasts.
