Fibroblast Derived Human Engineered Connective Tissue for Screening Applications
3.4K Views
•
09:50 min
•
August 20th, 2021
DOI :
August 20th, 2021
•Gabriela L. Santos1,2, Tim Meyer1,2, Malte Tiburcy1,2, Alisa DeGrave1,2, Wolfram-Hubertus Zimmermann1,2,3,4,5, Susanne Lutz1,2
1Institute of Pharmacology and Toxicology, University Medical Center Goettingen, 2DZHK (German Center for Cardiovascular Research) partner site, Goettingen, 3Cluster of Excellence “Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells” (MBExC), University of Goettingen, 4Center for Neurodegenerative Diseases (DZNE), 5Fraunhofer Institute for Translational Medicine and Pharmacology (ITMP)
Explore More Videos
Chapters in this video
0:04
Introduction
0:52
Primary Cardiac Fibroblast Preparation
1:39
Enzymatic Dispersion of Human Cardiac Fibroblasts
2:52
Engineered Connective Tissue Preparation
5:28
Cross-Sectional Area Measurement to Assess ECT Compaction
5:55
Pole Deflection Analysis to Monitor ECT Contraction
6:32
Destructive Tensile Measurement and Stress-Strain Analysis to Assess ECT
8:20
Results: Effect of Actin Polymerization on ECT Compaction, Contraction, and Stiffness
9:22
Conclusion
Related Videos
10:45
Organotypic Collagen I Assay: A Malleable Platform to Assess Cell Behaviour in a 3-Dimensional Context
22.5K Views
06:04
Murine Dermal Fibroblast Isolation by FACS
21.2K Views
12:13
Engineering Fibrin-based Tissue Constructs from Myofibroblasts and Application of Constraints and Strain to Induce Cell and Collagen Reorganization
10.9K Views
07:21
Fibroblast-Derived 3D Matrix System Applicable to Endothelial Tube Formation Assay
7.5K Views
09:24
Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets
14.5K Views
08:59
Human Dupuytren's Ex Vivo Culture for the Study of Myofibroblasts and Extracellular Matrix Interactions
10.5K Views
09:43
Databases to Efficiently Manage Medium Sized, Low Velocity, Multidimensional Data in Tissue Engineering
6.3K Views
09:23
Generation, High-Throughput Screening, and Biobanking of Human-Induced Pluripotent Stem Cell-Derived Cardiac Spheroids
3.8K Views
08:32
Engineering Tendon Assembloids to Probe Cellular Crosstalk in Disease and Repair
955 Views
06:51
Ultrasonic-augmented Primary Adult Fibroblast Isolation
7.6K Views