Institut de Biologie de l’École Normale Supérieure

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Selective Cleaning of Wild Caenorhabditis Nematodes to Enrich for Intestinal Microbiome Bacteria

Emily Morgan¹, Jonah Faye Longares¹, Marie-Anne Félix², Robert J. Luallen¹

¹Department of Biology, San Diego State University, ²Institut de Biologie de l’École Normale Supérieure

Wild Caenorhabditis nematodes are associated with many microbes, often in the gut lumen or infecting the intestine. This protocol details a method to enrich unculturable microbes colonizing the intestine, taking advantage of the resistance of the dauer cuticle.