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Institut de Biologie de l’École Normale Supérieure

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Immunology and Infection

Selective Cleaning of Wild Caenorhabditis Nematodes to Enrich for Intestinal Microbiome Bacteria
Emily Morgan 1, Jonah Faye Longares 1, Marie-Anne Félix 2, Robert J. Luallen 1
1Department of Biology, San Diego State University, 2Institut de Biologie de l’École Normale Supérieure

Wild Caenorhabditis nematodes are associated with many microbes, often in the gut lumen or infecting the intestine. This protocol details a method to enrich unculturable microbes colonizing the intestine, taking advantage of the resistance of the dauer cuticle.

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