<p class="jove_content">This protocol describes a method to establish a human blood-brain barrier (BBB) <em>in vitro</em> model. The endothelial cells and pericytes are seeded on each side of an insert filter (blood compartment), and astrocytes are seeded in the bottom well (brain compartment). The model characterized was used for nanoparticles transport experiments.</p>

a-triple-culture-cell-system-modeling-the-human-blood-brain-barrier

A Triple Culture Cell System Modeling the Human Blood-Brain Barrier

The delivery of drugs to the brain remains a challenge due to the blood-brain barrier's (BBB) highly specific and restrictive properties, which controls ...

Institution - Yamaguchi University Graduate School of Medicine, Department of Neurology and Clinical Neuroscience

Articles Published in JoVE