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A Triple Culture Cell System Modeling the Human Blood-Brain Barrier

3.4K Views

09:21 min

November 30th, 2021

DOI :

10.3791/63134-v

November 30th, 2021


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Triple Culture Cell System

Chapters in this video

0:04

Introduction

1:23

Seeding of Astrocytes in the Wells

2:50

Seeding of Pericytes on the Reverted Insert Filters

4:35

Seeding of Endothelial Cells on the Insert Filters

6:00

Maintenance of the Triple Cell Culture for the Induction of the BBB Properties

6:35

Results: Properties of the Triple Culture BBB Model and the Comparison of BLEC Gene Expression and Protein Level of Distinctive Markers in the Triple Culture Model with the Co‐culture BBB Model

8:49

Conclusion

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