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A Triple Culture Cell System Modeling the Human Blood-Brain Barrier

DOI :

10.3791/63134-v

9:21 min

November 30th, 2021

November 30th, 2021

2,358 Views

1Univ. Artois, UR 2465, Laboratoire de la Barrière Hémato-Encéphalique (LBHE), 2Queen Mary University of London, Department of Chemistry, 3Yamaguchi University Graduate School of Medicine, Department of Neurology and Clinical Neuroscience

This protocol describes a method to establish a human blood-brain barrier (BBB) in vitro model. The endothelial cells and pericytes are seeded on each side of an insert filter (blood compartment), and astrocytes are seeded in the bottom well (brain compartment). The model characterized was used for nanoparticles transport experiments.

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Triple Culture Cell System

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