A Triple Culture Cell System Modeling the Human Blood-Brain BarrierEleonora Rizzi *1, Clémence Deligne *1, Lucie Dehouck 1, Roberta Bilardo 2, Yasutero Sano 3, Fumitaka Shimizu 3, Takashi Kanda 3, Marina Resmini 2, Fabien Gosselet 1, Marie-Pierre Dehouck 1, Caroline Mysiorek 1
1Univ. Artois, UR 2465, Laboratoire de la Barrière Hémato-Encéphalique (LBHE), 2Queen Mary University of London, Department of Chemistry, 3Yamaguchi University Graduate School of Medicine, Department of Neurology and Clinical Neuroscience
This protocol describes a method to establish a human blood-brain barrier (BBB) in vitro model. The endothelial cells and pericytes are seeded on each side of an insert filter (blood compartment), and astrocytes are seeded in the bottom well (brain compartment). The model characterized was used for nanoparticles transport experiments.
