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Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health

5 ARTICLES PUBLISHED IN JoVE

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Biology

Paraffin-Embedded and Frozen Sections of Drosophila Adult Muscles
Mariya M. Kucherenko 1, April K. Marrone 1, Valentyna M. Rishko 1, Andriy S. Yatsenko 1, Annekatrin Klepzig 1, Halyna R. Shcherbata 1
1Gene Expression and Signaling Research Group, Max Planck Institute for Biophysical Chemistry

Identification of mechanisms underlying muscle damage is crucial. Here we present the histological technique for preparing paraffin-embedded and frozen sections of Drosophila thoracic muscles. This allows analysis of muscle morphology and localization of protein and other muscle cell components.

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Medicine

In vivo 19F MRI for Cell Tracking
Mangala Srinivas 1, Philipp Boehm-Sturm 2, Markus Aswendt 2, Eberhard D. Pracht 2,3, Carl G. Figdor 1, I. Jolanda de Vries 1, Mathias Hoehn 2
1Department of Tumor Immunology, Nijmegen Center for Molecular Life Sciences, Radboud University Medical Center, 2In-Vivo-NMR Laboratory, Max Planck Institute for Neurological Research, 3German Center for Neurodegenerative Diseases (DZNE)

We describe a general protocol for in vivo cell tracking using MRI in a mouse model with ex vivo labeled cells. A typical protocol for cell labeling, image acquisition processing and quantification is included.

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Biology

Measurement of Metabolic Rate in Drosophila using Respirometry
Andriy S. Yatsenko 1, April K. Marrone 1, Mariya M. Kucherenko 1, Halyna R. Shcherbata 1
1Max Planck Research Group of Gene Expression and Signaling, Max Planck Institute for Biophysical Chemistry

Metabolic disorders are among one of the most common diseases in humans. The genetically tractable model organism D. melanogaster can be used to identify novel genes that regulate metabolism. This paper describes a relatively simple method which allows studying the metabolic rate in flies by measuring their CO2 production.

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Neuroscience

Endovascular Perforation Model for Subarachnoid Hemorrhage Combined with Magnetic Resonance Imaging (MRI)
Shuheng Liu *1, Katharina Tielking *1, Dario von Wedel 1, Melina Nieminen-Kelhä 1, Susanne Mueller 2,3, Philipp Boehm-Sturm 2,3, Peter Vajkoczy 1, Ran Xu 1
1Department of Neurosurgery, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, and Humboldt-Universität zu Berlin, and Berlin Institute of Health, 2Department of Experimental Neurology and Center for Stroke Research Berlin, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 3NeuroCure Cluster of Excellence and Charité Core Facility 7T Experimental MRIs, Charité - Universitätsmedizin Berlin

Here we present a standardized SAH mouse model, induced by endovascular filament perforation, combined with magnetic resonance imaging (MRI) 24 h after operation to ensure the correct bleeding site and exclude other relevant intracranial pathologies.

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Medicine

A Model of Reverse Vascular Remodeling in Pulmonary Hypertension Due to Left Heart Disease by Aortic Debanding in Rats
Pengchao Sang 1,2,3, Mariya M. Kucherenko 1,2,3, Juquan Yao 2, Qiuhua Li 2, Szandor Simmons 2,3, Wolfgang M. Kuebler *2,3, Christoph Knosalla *1,3,4
1Department of Cardiothoracic and Vascular Surgery, German Heart Center Berlin (DHZB), 2Institute of Physiology, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 3DZHK (German Centre for Cardiovascular Research), Partner Site Berlin, 4Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health

The present protocol describes a surgical procedure to remove ascending-aortic banding in a rat model of pulmonary hypertension due to left heart disease. This technique studies endogenous mechanisms of reverse remodeling in the pulmonary circulation and the right heart, thus informing strategies to reverse pulmonary hypertension and/or right ventricular dysfunction.

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