JAIST Venture Business Laboratory, Ishikawa Create Labo

1 ARTICLES PUBLISHED IN JoVE

Bioengineering

A Nonsequencing Approach for the Rapid Detection of RNA Editing

Ruchika ¹, Toshifumi Tshukahara¹, Manish Biyani^1,2

¹Department of Bioscience and Biotechnology, Japan Advanced Institute of Science and Technology, ²BioSeeds Corporation, JAIST Venture Business Laboratory, Ishikawa Create Labo

Rapid detection and reliable quantification of RNA editing events at a genomic scale remain challenging and currently rely on direct RNA sequencing methods. The protocol described here uses microtemperature gradient gel electrophoresis (µTGGE) as a simple, quick, and portable method of detecting RNA editing.