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A Nonsequencing Approach for the Rapid Detection of RNA Editing

DOI :

10.3791/63591-v

April 21st, 2022

April 21st, 2022

2,346 Views

1Department of Bioscience and Biotechnology, Japan Advanced Institute of Science and Technology, 2BioSeeds Corporation, JAIST Venture Business Laboratory, Ishikawa Create Labo

Rapid detection and reliable quantification of RNA editing events at a genomic scale remain challenging and currently rely on direct RNA sequencing methods. The protocol described here uses microtemperature gradient gel electrophoresis (µTGGE) as a simple, quick, and portable method of detecting RNA editing.

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