Sanjay Gandhi Postgraduate Institute of Medical Sciences

2 ARTICLES PUBLISHED IN JoVE

Genetics

Reverse Genetics to Engineer Positive-Sense RNA Virus Variants

Shalini Soni¹, Shubhra Agarwal¹, Rakesh Aggarwal², Naga Suresh Veerapu¹

¹Virology Section, Department of Life Sciences, Shiv Nadar University, ²Gastroenterology, Sanjay Gandhi Postgraduate Institute of Medical Sciences

The protocol describes a method for introducing controllable genetic diversity in the hepatitis C virus genome by combining full-length mutant RNA synthesis using error-prone PCR and reverse genetics. The method provides a model for phenotype selection and can be used for 10 kb long positive-sense RNA virus genomes.

Biology

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics

Anamika Singh^1,2, Mohd Adnan Siddiqui¹, Afzal Azim³, Neeraj Sinha^1,2

¹Centre of Biomedical Research, SGPGIMS Campus, ²Academy of Scientific and Innovative Research (AcSIR), ³Department of Critical Care Medicine, Sanjay Gandhi Postgraduate Institute of Medical Sciences

Nuclear magnetic resonance (NMR) spectroscopy is used to identify dysregulation in the metabolites in patients with various diseases. This technique allows the quantification of the deranged metabolites, unraveling the pathophysiological insights. Here, we describe the step-by-step procedure of the NMR-based approach for the metabolic characterization of the patients.