Chemical Triphosphorylation of OligonucleotidesGrant A. L. Bare 1, David P. Horning 1
1The Salk Institute
Oligonucleotide 5′-triphosphates are ubiquitous components in essential biological pathways and have seen increasing use in biotechnology applications. Here, we describe techniques for the routine synthesis and purification of oligonucleotide 5′-triphosphates, starting from oligonucleotides prepared by standard automated synthesis techniques.