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Chemical Triphosphorylation of Oligonucleotides

DOI :

10.3791/63877-v

13:19 min

June 2nd, 2022

June 2nd, 2022

2,200 Views

1The Salk Institute

Oligonucleotide 5′-triphosphates are ubiquitous components in essential biological pathways and have seen increasing use in biotechnology applications. Here, we describe techniques for the routine synthesis and purification of oligonucleotide 5′-triphosphates, starting from oligonucleotides prepared by standard automated synthesis techniques.

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Triphosphorylation

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