Groupe Hospitalier Paris Saint Joseph

4 ARTICLES PUBLISHED IN JoVE

Medicine

Chronic Thromboembolic Pulmonary Hypertension and Assessment of Right Ventricular Function in the Piglet

Pierre-Emmanuel Noly¹, Julien Guihaire^1,4, Matthieu Coblence¹, Peter Dorfmüller^2,5, Elie Fadel^1,3, Olaf Mercier^1,3

¹Surgical Research Lab, Marie Lannelongue Hospital, ²Department of Pathology, Marie Lannelongue Hospital, ³Department of Thoracic and Vascular Surgery, Marie Lannelongue Hospital, ⁴Thoracic and Cardiovascular Surgery, University Hospital of Rennes, ⁵INSERM U999 Paris-Sud University

Chronic Thromboembolic Pulmonary Hypertension (CTEPH) and Right Ventricular (RV) dysfunction were induced in piglets by progressive obstruction of the pulmonary arteries. Consequences were remarkably similar to those observed in CTEPH patients. This animal model would be a very useful tool for pathophysiology and therapeutic experiments on CTEPH and RV failure.

Medicine

Induction and Phenotyping of Acute Right Heart Failure in a Large Animal Model of Chronic Thromboembolic Pulmonary Hypertension

David Boulate¹, Myriam Amsallem^1,2, Jean-Baptiste Menager¹, Simon Dang Van¹, Peter Dorfmuller³, Andrew Connolly⁴, Alban Todesco⁵, Benoit Decante¹, Elie Fadel¹, Francois Haddad², Olaf Mercier¹

¹Research and Innovation Unit, RHU BioArt Lung 2020, Marie Lannelongue Hospital, ²Phenotypic and Biomarker Core Laboratory, Cardiovascular Institute, Stanford University, ³Department of pathology, Marie Lannelongue Hospital, ⁴Department of pathology, UCSF School of Medicine, ⁵Department of thoracic surgery, Hopital Nord, APHM, Aix-Marseille University

We present a protocol to induce and phenotype an acute right heart failure in a large animal model with chronic pulmonary hypertension. This model can be used to test therapeutic interventions, to develop right heart metrics or to improve the understanding of acute right heart failure pathophysiology.

Medicine

Functional Assessment of the Donor Heart During Ex Situ Perfusion: Insights from Pressure-Volume Loops and Surface Echocardiography

Simon Dang Van^1,2,3, Dorothée Brunet^1,2,3, Ali Akamkam^1,2,3, Benoit Decante^1,2, Julien Guihaire^1,2,3

¹Laboratory of Preclinical Research, Department of Research and Innovation, Groupe Hospitalier Paris Saint Joseph, ²Inserm UMR S 999, Pulmonary Hypertension: Pathophysiology and Novel Therapies, Marie Lannelongue Hospital, Groupe Hospitalier Paris Saint Joseph, ³Department of Cardiac Surgery, Marie Lannelongue Hospital

A reliable noninvasive approach for functional assessment of the donor heart during normothermic ex situ heart perfusion (NESP) is lacking. We herein describe a protocol for ex situ assessment of myocardial performance using the epicardial echocardiography and conductance catheter method.

Medicine

Coronary Angiography During Ex-Situ Heart Perfusion in a Porcine Model

Ali Akamkam^1,2,3, Vincenzo Palermo⁴, Tiphaine Legrand⁵, Guillaume Fadel^2,3,6, Benoît Decante^2,3, Simon Dang Van^2,3,7, Jean-Noel Andarelli⁴, Valérie Furlan⁵, Julien Guihaire^1,2,3,8

¹Department of Cardiovascular Surgery, Marie Lannelongue Hospital, Groupe Hospitalier Paris Saint Joseph, ²Laboratory of Preclinical Research, Department of Research and Innovation, Groupe Hospitalier Paris Saint Joseph, ³Inserm UMR S 999, Pulmonary Hypertension: Pathophysiology and Novel Therapies, Marie Lannelongue Hospital, Groupe Hospitalier Paris Saint Joseph, ⁴Department of Cardiology, Marie Lannelongue Hospital, Groupe Hospitalier Paris Saint Joseph, ⁵Department of Pharmacology and Toxicology, AP-HP. University of Paris-Saclay, ⁶Department of Thoracic Surgery, Marie Lannelongue Hospital, Groupe Hospitalier Paris Saint Joseph, ⁷Department of Cardiovascular Surgery, University Hospital of Angers, ⁸University of Paris Saclay

Screening for coronary artery disease could increase the procurement of donor hearts with extended criteria. A protocol for performing reproducible coronary angiography during ex-situ heart perfusion is described herein in a porcine model.