UF/IFAS Tropical Research and Education Center

3 ARTICLES PUBLISHED IN JoVE

Biology

Repeatable Stair-step Assay to Access the Allelopathic Potential of Weedy Rice (Oryza sativa ssp.)

Brooklyn C. Schumaker¹, Shandrea Stallworth¹, Edicarlos De Castro¹, Mary Grace Fuller¹, Swati Shrestha¹, Te-Ming Tseng¹

¹Department of Plant and Soil Science, Mississippi State University

Allelopathy has shown promise as a useful supplemental weed control strategy in cropping systems. To determine the allelopathic potential of a desired plant specimen, a stair-step screening method is provided.

Biology

A Contrast of Three Inoculation Techniques used to Determine the Race of Unknown Fusarium oxysporum f.sp. niveum Isolates

James C. Fulton^*1, Matthew A. Cullen^*1, Kristin Beckham¹, Tatiana Sanchez², Zhuxuan Xu¹, Preston Stern¹, Gary Vallad³, Geoffrey Meru^4,5, Cecilia McGregor⁶, Nicholas S. Dufault¹

¹Department of Plant Pathology, University of Florida, ²University of Florida Institute of Food and Agricultural Sciences, ³Gulf Coast Research and Education Center, University of Florida, ⁴Horticultural Sciences Department, University of Florida, ⁵Tropical Research and Education Center, University of Florida, ⁶Department of Horticulture, University of Georgia

Managing Fusarium wilt of watermelon requires knowledge of the pathogen races present. Here, we describe the root-dip, infested kernel seeding, and modified tray-dip inoculation methods to demonstrate their efficacy in race-typing of the pathogenic fungus Fusarium oxysporum f. sp niveum (Fon).

Biology

Embryo Rescue Protocol for Interspecific Hybridization in Squash

Yuqing Fu¹, Swati Shrestha¹, Pamela Moon¹, Geoffrey Meru¹

¹UF/IFAS Tropical Research and Education Center

The article describes an embryo rescue protocol for the regeneration of immature embryos derived from the interspecific hybridization of Cucurbita pepo and Cucurbita moschata. The protocol can be easily replicated and will be an important resource for squash breeding programs.