A Contrast of Three Inoculation Techniques used to Determine the Race of Unknown Fusarium oxysporum f.sp. niveum Isolates

3.1K Views

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11:48 min

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October 28th, 2021

DOI :

10.3791/63181-v

October 28th, 2021

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James C. Fulton*¹, Matthew A. Cullen*¹, Kristin Beckham¹, Tatiana Sanchez², Zhuxuan Xu¹, Preston Stern¹, Gary Vallad³, Geoffrey Meru⁴^,⁵, Cecilia McGregor⁶, Nicholas S. Dufault¹

¹Department of Plant Pathology, University of Florida, ²University of Florida Institute of Food and Agricultural Sciences, ³Gulf Coast Research and Education Center, University of Florida, ⁴Horticultural Sciences Department, University of Florida, ⁵Tropical Research and Education Center, University of Florida, ⁶Department of Horticulture, University of Georgia

Summary

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Fusarium Oxysporum

Inoculation Techniques

Integrated Disease Management

Seed Germination

Conidia Quantification

Plant Health Practitioners

Inoculum Solution

Chapters in this video

0:04

Introduction

0:52

Planting Conditions

1:49

Prepare the Inoculum and Inoculate the Plant Roots

5:00

Determining Race by Infested Kernel Method (IKM)

7:04

Combine Soil and Grain

8:04

Determining Race by Modified Tray-Dip Method (MTDM) and Disease Rating

10:08

Results: Phenotypic Results of Race Identification Methods

10:56

Conclusion

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