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Skeletal Muscle Gender Dimorphism from Proteomics

DOI :

10.3791/3536-v

9:29 min

December 14th, 2011

December 14th, 2011

12,139 Views

1Center for Proteomics, Smith College, 2Department of Molecular Biophysics and Biochemistry, Yale University, 3Department of Chemistry, Smith College, 4Department of Biological Sciences and Center for Proteomics, Smith College

A straight-forward set of methods to isolate and determine the identity of the most abundant proteins expressed in skeletal muscle. About 800 spots are discerned on a two-dimensional gel from 10 mg muscle; this allows for the determination of gender-specific protein expression. These methods will give equivalent results in most tissues.

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Skeletal Muscle

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