Uniformed Services University and Walter Reed National Military Medical Center

1 ARTICLES PUBLISHED IN JoVE

Cancer Research

Industrialized, Artificial Intelligence-guided Laser Microdissection for Microscaled Proteomic Analysis of the Tumor Microenvironment

Dave Mitchell^*1,2, Allison L. Hunt^*1,4, Kelly A. Conrads^1,2, Brian L. Hood^1,2, Sasha C. Makohon-Moore^1,2, Christine Rojas¹, G. Larry Maxwell^1,3,4, Nicholas W. Bateman^1,2,3, Thomas P. Conrads^1,3,4

¹Women’s Health Integrated Research Center, Gynecologic Cancer Center of Excellence, Department of Obstetrics and Gynecology, Uniformed Services University and Walter Reed National Military Medical Center, ²The Henry M. Jackson Foundation for the Advancement of Military Medicine, ³The John P. Murtha Cancer Center Research Program, Department of Surgery, Uniformed Services University, ⁴Women’s Health Integrated Research Center, Inova Women’s Service Line, Inova Health System

This protocol describes a high-throughput workflow for artificial intelligence-driven segmentation of pathology-confirmed regions of interest from stained, thin tissue section images for enrichment of histology-resolved cell populations using laser microdissection. This strategy includes a novel algorithm enabling the transfer of demarcations denoting cell populations of interest directly to laser microscopes.