Industrialized, Artificial Intelligence-guided Laser Microdissection for Microscaled Proteomic Analysis of the Tumor Microenvironment
3.5K Views
•
13:01 min
•
June 3rd, 2022
DOI :
June 3rd, 2022
•Dave Mitchell*1,2, Allison L. Hunt*1,4, Kelly A. Conrads1,2, Brian L. Hood1,2, Sasha C. Makohon-Moore1,2, Christine Rojas1, G. Larry Maxwell1,3,4, Nicholas W. Bateman1,2,3, Thomas P. Conrads1,3,4
1Women’s Health Integrated Research Center, Gynecologic Cancer Center of Excellence, Department of Obstetrics and Gynecology, Uniformed Services University and Walter Reed National Military Medical Center, 2The Henry M. Jackson Foundation for the Advancement of Military Medicine, 3The John P. Murtha Cancer Center Research Program, Department of Surgery, Uniformed Services University, 4Women’s Health Integrated Research Center, Inova Women’s Service Line, Inova Health System
Chapters in this video
0:04
Introduction
1:07
LMD Slide(s) Preparation
1:58
Automated Feature Selection Using Image Analysis Software
6:58
Laser Microdissectionvvv
9:41
Protein Digestion by Pressure-Cycle Technology (PCT) and Sample Analysis Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
10:55
Results: Analysis of the Proteins in LMD-Enriched and Whole Tumor Samples
12:09
Conclusion
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