Study of the Functions and Activities of Neuronal K-Cl Co-Transporter KCC2 Using Western BlottingSunday Solomon Josiah 1, Nur Farah Meor Azlan 1, Asami Oguro-Ando 1, Jinwei Zhang 1
1Institute of Biomedical and Clinical Sciences, Medical School, Faculty of Health and Life Sciences, University of Exeter, Hatherly Laboratories
The present protocol highlights the application of western blotting technique to study the functions and activities of neuronal K-Cl co-transporter KCC2. The protocol describes the investigation of KCC2 phosphorylation at kinase regulatory sites Thr906/1007 via western blotting. Also, additional methods to confirm KCC2 activity are briefly highlighted in this text.
