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Study of the Functions and Activities of Neuronal K-Cl Co-Transporter KCC2 Using Western Blotting

2.0K Views

10:08 min

December 9th, 2022

DOI :

10.3791/64179-v

December 9th, 2022


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K Cl Co transporter

Chapters in this video

0:05

Introduction

0:50

Cell Culture and Transfection

2:28

Preparation of Cell Lysates and Loading Samples

3:11

Preparation of Immunoprecipitants from Cell Lysates

4:40

Performing the Western Blot

6:23

Antibody Staining, Image Development, Image Acquisition, and Data Quantification

7:16

Results: Western Blotting Technique to Study the Functions and Activities of Neuronal K-Cl Co-Transporter KCC2

9:25

Conclusion

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