University of Prince Edward Island

2 ARTICLES PUBLISHED IN JoVE

Medicine

Early Pathological and Magnetic Resonance Detection of Cerebral Injury Using a Rat Model of Neonatal Hypoxic Ischemic Encephalopathy

Michael G. Melek¹, Rheal Towner^2,3, Johannes Kung¹, Debra Saunders⁴, Michelle Zales^3,4, Faizah Bhatti^1,3

¹Department of Pediatrics, University of Oklahoma Health Sciences Center, ²Department of Chemistry, University of Prince Edward Island, ³Center for Neuroscience, University of Oklahoma Health Sciences Center, ⁴Oklahoma Medical Research Foundation

The present protocol describes a rodent model of newborn hypoxic-ischemic injury for identifying early changes in cerebral tissue by gross morphology and magnetic resonance imaging. This has benefits over existing models, which can be used to study late injury but do not allow the evaluation of reproducible early changes.

Biochemistry

Bioassay-Guided Identification of Natural Products for Biocontrol by Thin Layer Chromatography-Direct Bioautography

Leah Gauthier^1,2, Brian D. Wagner², Sue Boyetchko³, Christopher W. Kirby^1,2

¹Charlottetown Research and Development Centre, Agriculture and Agri-Food Canada, ²Department of Chemistry, University of Prince Edward Island, ³Saskatoon Research and Development Centre, Agriculture and Agri-Food Canada

We describe the use of thin-layer chromatography direct bioautography assay and liquid chromatography-mass spectrometry to identify microbial natural products that display antagonism against fungal pathogens using the pathogen Sclerotinia sclerotiorum and biopesticidal Bacillus isolates as a model system.