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Third Military Medical University of Chinese PLA

5 ARTICLES PUBLISHED IN JoVE

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Engineering

Free-form Light Actuators — Fabrication and Control of Actuation in Microscopic Scale
Hao Zeng 1, Piotr Wasylczyk 2, Camilla Parmeggiani 1,3, Daniele Martella 1,4, Diederik Sybolt Wiersma 1
1European Labratory for Non-Linear Spectroscopy, University of Florence, 2Institute of Experimental Physics, Faculty of Physics, University of Warsaw, 3CNR-INO, 4Chemistry Department, University of Florence

Here, we fabricate 3D polymeric micro/nano structures in which both the shape and the molecular alignment can be engineered with nanometer scale accuracy by the use of direct laser writing. Light induced deformation of several types of liquid crystalline elastomer microstructures can be controlled in the microscopic scale.

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Biology

A Quantitative Dot Blot Assay for AAV Titration and Its Use for Functional Assessment of the Adeno-associated Virus Assembly-activating Proteins
John M. Powers 1, Xiao Lan Chang 1, Zhen Song 1, Hiroyuki Nakai 1,2
1Department of Molecular and Medical Genetics, Oregon Health & Science University, 2Department of Molecular Microbiology and Immunology, Oregon Health & Science University

This manuscript details a straightforward dot blot assay for quantitation of adeno-associated virus (AAV) titers and its application to study the role of assembly-activating proteins (AAPs), a novel class of non-structural viral proteins found in all AAV serotypes, in promoting the assembly of capsids derived from cognate and heterologous AAV serotypes.

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Immunology and Infection

Efficient Transfection of In vitro Transcribed mRNA in Cultured Cells Using Peptide-Poloxamine Nanoparticles
Qin Xiao *1, Yuheng Liu *1, Dandan Zhang 1, Chao Li 1, Qihua Yang 1, Dongshui Lu 1, Weijun Zhang 1, Joseph Rosenecker 2, Quanming Zou 1, Yang Li 3, Shan Guan 1
1National Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, Third Military Medical University, 2Department of Pediatrics, Ludwig-Maximilians University of Munich, 3Department of Pharmacy, Southwest Hospital, Third Military Medical University

A self-assembled peptide-poloxamine nanoparticle (PP-sNp) is developed using a microfluidic mixing device to encapsulate and deliver in vitro transcribed messenger RNA. The described mRNA/PP-sNp could efficiently transfect cultured cells in vitro.

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Immunology and Infection

In Vitro Cellular Activity Evaluation of the Nanoemulsion Vaccine Adjuvant Ophiopogonin D
Xing Luo 1, Yanan Tong 1, Xiaoqiang Zeng 1, Yan Ye 1, Yun Yang 1, Zhen Song 1, Zelong Zhang 1, Haibo Li 1, Jining Gao 2, Xuhu Mao 1, Hao Zeng 1, Quanming Zou 1, Hongwu Sun 1
1Department of Microbiology and Biochemical Pharmacy, National Engineering Research Centre of Immunological Products, College of Pharmacy, Third Military Medical University of Chinese PLA, 2Institute of Combined Injury of PLA, College of Military Preventive Medicine, Third Military Medical University of Chinese PLA

The protocol presents detailed methods for evaluating whether the nanoemulsion ophiopogonin D adjuvant promotes effective cellular immune responses.

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Immunology and Infection

Preparation, Characteristics, Toxicity, and Efficacy Evaluation of the Nasal Self-Assembled Nanoemulsion Tumor Vaccine In Vitro and In Vivo
Zelong Zhang 1, Dingyi Cai 1, Shuang Ge 1, Xing Luo 1, Xiaoqiang Zeng 1, Yan Ye 1, Zhen Song 1, Liusheng Peng 1, Haibo Li 1, Quanming Zou 1, Hao Zeng 1, Hongwu Sun 1, Yun Yang 1
1National Engineering Research Centre of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Third Military Medical University of Chinese PLA

Here, we present detailed methods for the preparation and evaluation of the nasal self-assembled nanoemulsion tumor vaccine in vitro and in vivo.

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