A Quantitative Dot Blot Assay for AAV Titration and Its Use for Functional Assessment of the Adeno-associated Virus Assembly-activating Proteins
13.6K Views
•
14:49 min
•
June 12th, 2018
DOI :
June 12th, 2018
•Chapters in this video
0:04
Title
0:46
Production of AAV in HEK 293 Cells by Plasmid DNA Transfection
2:50
Recovery of Viral Particles, and Treatment with Serratia marcescens Endonuclease and Proteinase K
4:20
Phenol-choloroform Extraction, Ethanol Precipitation, and Preparation of Plasmid DNA Standards
5:55
Dot Blot Assay
8:46
Hybridization and Washing
10:50
Results: AAV Titration and Functional Assessment of AAP Proteins Using a Quantitative Dot Blot Assay
12:58
Conclusion
Related Videos
The Ladder Rung Walking Task: A Scoring System and its Practical Application.
25.9K Views
The Microfluidic Probe: Operation and Use for Localized Surface Processing
8.8K Views
Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
29.9K Views
Multiplex PCR and Reverse Line Blot Hybridization Assay (mPCR/RLB)
43.4K Views
A Quantitative Assay for Insulin-expressing Colony-forming Progenitors
13.4K Views
Use of a Robot for High-throughput Crystallization of Membrane Proteins in Lipidic Mesophases
17.5K Views
A Hybrid DNA Extraction Method for the Qualitative and Quantitative Assessment of Bacterial Communities from Poultry Production Samples
14.4K Views
A Protocol for Using Gene Set Enrichment Analysis to Identify the Appropriate Animal Model for Translational Research
17.7K Views
Immunofluorescent Labeling of Plant Virus and Insect Vector Proteins in Hemipteran Guts
1.6K Views
Process Development for the Production and Purification of Adeno-Associated Virus (AAV)2 Vector using Baculovirus-Insect Cell Culture System
9.0K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved