JoVE Logo

Sign In

MSD R&D Innovation Centre

1 ARTICLES PUBLISHED IN JoVE

image

Neuroscience

Fluorescence-Activated Nuclei Negative Sorting of Neurons Combined with Single Nuclei RNA Sequencing to Study the Hippocampal Neurogenic Niche
Thomas Kerloch 1, Tjaša Lepko 2, Kirill Shkura 2, François Guillemot 1, Sébastien Gillotin 2
1The Francis Crick Institute, 2MSD R&D Innovation Centre

Presented here is a method to sequence single nuclei isolated from the mouse dentate gyrus that excludes most neurons through fluorescence-activated nuclei (FAN)-sorting. This approach generates high-quality expression profiles and facilitates the study of most other cell types represented in the niche, including scarce populations such as neural stem cells.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved