JoVE Journal

Neuroscience

This content is Free Access.

Fluorescence-Activated Nuclei Negative Sorting of Neurons Combined with Single Nuclei RNA Sequencing to Study the Hippocampal Neurogenic Niche

Presented here is a method to sequence single nuclei isolated from the mouse dentate gyrus that excludes most neurons through fluorescence-activated nuclei (FAN)-sorting. This approach generates high-quality expression profiles and facilitates the study of most other cell types represented in the niche, including scarce populations such as neural stem cells.

Chapters in this video

0:04

Introduction

1:08

Dissection of the Dentate Gyrus (DG)

1:53

Tissue Dissociation, Single Nuclei Isolation, and Anti-NeuN Immunostaining

4:03

Fluorescence Activated Nuclei Sorting (FANS) to Exclude Neuronal Populations

5:12

Preparation of the Single Nuclei Suspension to Perform Single Nuclei RNA Sequencing

5:59

Results: Preparation of a Suspension of Non-Neuronal Single Nuclei Isolated from the Dentate Gyrus (DG)

7:51

Conclusion

Related Videos

We use cookies to enhance your experience on our website.

By continuing to use our website or clicking “Continue”, you are agreeing to accept our cookies.

Learn More