Fluorescence-Activated Nuclei Negative Sorting of Neurons Combined with Single Nuclei RNA Sequencing to Study the Hippocampal Neurogenic Niche
2.6K Views
•
08:16 min
•
October 20th, 2022
DOI :
October 20th, 2022
•Chapters in this video
0:04
Introduction
1:08
Dissection of the Dentate Gyrus (DG)
1:53
Tissue Dissociation, Single Nuclei Isolation, and Anti-NeuN Immunostaining
4:03
Fluorescence Activated Nuclei Sorting (FANS) to Exclude Neuronal Populations
5:12
Preparation of the Single Nuclei Suspension to Perform Single Nuclei RNA Sequencing
5:59
Results: Preparation of a Suspension of Non-Neuronal Single Nuclei Isolated from the Dentate Gyrus (DG)
7:51
Conclusion
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