The present protocol assesses the locomotor activity of Drosophila by tracking and analyzing the movement of flies in a hand-made arena using open-source software Fiji, compatible with plugins to segment pixels of each frame based on high-definition video recording to calculate parameters of speed, distance, etc.
The protocol introduces a novel mini-open TLIF, which can significantly reduce intraoperative blood loss, achieving minimally invasive outcomes with enhanced recovery.
This protocol presents the management for gastroesophageal varices (GEVs) with endoscopic injection sclerotherapy assisted by cyanoacrylate and clips (CISC), which can improve the effectiveness of sclerotherapy and reduce complications.
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