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A Simple Technique to Assay Locomotor Activity in Drosophila

DOI :

10.3791/65092-v

February 24th, 2023

February 24th, 2023

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1Institute of Neuroscience, Soochow University, 2Department of Neurology and Clinical Research Center of Neurological Disease, The Second Affiliated Hospital of Soochow University

The present protocol assesses the locomotor activity of Drosophila by tracking and analyzing the movement of flies in a hand-made arena using open-source software Fiji, compatible with plugins to segment pixels of each frame based on high-definition video recording to calculate parameters of speed, distance, etc.

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