Multiphoton Intravital Imaging for Monitoring Leukocyte Recruitment during Arteriogenesis in a Murine Hindlimb Model

1.3K Views

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07:50 min

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September 30th, 2021

DOI :

10.3791/62969-v

September 30th, 2021

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Manuel Lasch¹^,²^,³, Mykhailo Vladymyrov⁴, Dominic van den Heuvel¹^,⁵, Philipp Götz¹^,³, Elisabeth Deindl¹^,³, Hellen Ishikawa-Ankerhold¹^,⁵

¹Walter-Brendel-Centre of Experimental Medicine, University Hospital, Ludwig-Maximilians-Universität München, ²Department of Otorhinolaryngology, Head & Neck Surgery, University Hospital, Ludwig-Maximilians-Universität München, ³Biomedical Center, Institute of Cardiovascular Physiology and Pathophysiology, Faculty of Medicine, Ludwig-Maximilians-Universität München, ⁴TKI, University of Bern, ⁵Department of Internal Medicine I and Cardiology, University Hospital, Ludwig-Maximilians-Universität München

Summary

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Multiphoton Intravital Imaging

Leukocyte Recruitment

Arteriogenesis

Murine Hindlimb Model

Multiphoton Microscopy

Immune Cell Adherence

Extravasation

Live Imaging

Spatiotemporal Resolution

Anesthetized Mouse

Surgical Procedure

Optical Coupling

Titanium Sapphire Laser

3D Scan Timelapse

Epifluorescence Microscope

Chapters in this video

0:05

Introduction

1:02

Tissue Preparation

2:18

Intravital Multiphoton Microscopy

6:12

Results: Implications of VivoFollow Drift Correction Software

7:05

Conclusion

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