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Florida Institute of Technology

2 ARTICLES PUBLISHED IN JoVE

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Environment

Standardized Methods for Measuring Induction of the Heat Shock Response in Caenorhabditis elegans
Nicole L. Golden 1, Rosemary N. Plagens 1, Karen S. Kim Guisbert 1, Eric Guisbert 1
1Department of Biomedical and Chemical Engineering and Sciences, Florida Institute of Technology

Here, standardized protocols are presented to assess induction of the heat shock response (HSR) in Caenorhabditis elegans using RT-qPCR at the molecular level, fluorescent reporters at the cellular level, and thermorecovery at the organismal level.

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Developmental Biology

In Vivo Visualization of Calcium Transients during Fertilization and Early Development in C. elegans
Michaela Foley 1, Katie Toperzer 1, Sara Sornat 1, Jesse Morgan 1, Savannah Brennan 1, Eric Guisbert 1, Karen Kim Guisbert 1
1Florida Institute of Technology

Here, we present protocols and tools for visualizing calcium during fertilization and early embryogenesis using a genetically encoded calcium reporter expressed in the germline of the model nematode C. elegans.

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