Photonics Group,
Department of Physics,
Photonics Group, Department of Physics
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Imaging immune surveillance by T cells and NK cells.
Immunological reviews Nov, 2002 | Pubmed ID: 12445274
Imaging the environment of green fluorescent protein.
Biophysical journal Dec, 2002 | Pubmed ID: 12496126
Fluorescence lifetime imaging of unstained tissues: early results in human breast cancer.
The Journal of pathology Mar, 2003 | Pubmed ID: 12579532
Studying biological tissue with fluorescence lifetime imaging: microscopy, endoscopy, and complex decay profiles.
Applied optics Jun, 2003 | Pubmed ID: 12790450
Time-resolved fluorescence anisotropy imaging applied to live cells.
Optics letters Mar, 2004 | Pubmed ID: 15035478
Holographic optical coherence imaging of rat osteogenic sarcoma tumor spheroids.
Applied optics Sep, 2004 | Pubmed ID: 15449473
Fluorescence imaging of two-photon linear dichroism: cholesterol depletion disrupts molecular orientation in cell membranes.
Biophysical journal Jan, 2005 | Pubmed ID: 15520272
Time-resolved fluorescence microscopy.
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology Jan, 2005 | Pubmed ID: 15616687
Quantitative 3D mapping of fluidic temperatures within microchannel networks using fluorescence lifetime imaging.
Analytical chemistry Apr, 2006 | Pubmed ID: 16579608
Fluorescence lifetime imaging provides enhanced contrast when imaging the phase-sensitive dye di-4-ANEPPDHQ in model membranes and live cells.
Biophysical journal Jun, 2006 | Pubmed ID: 16617080
Microclusters of inhibitory killer immunoglobulin-like receptor signaling at natural killer cell immunological synapses.
The Journal of cell biology Jul, 2006 | Pubmed ID: 16801390
Structurally distinct membrane nanotubes between human macrophages support long-distance vesicular traffic or surfing of bacteria.
Journal of immunology (Baltimore, Md. : 1950) Dec, 2006 | Pubmed ID: 17142745
Tissue characterization using dimensionality reduction and fluorescence imaging.
Medical image computing and computer-assisted intervention : MICCAI ... International Conference on Medical Image Computing and Computer-Assisted Intervention , 2006 | Pubmed ID: 17354820
Rapid hyperspectral fluorescence lifetime imaging.
Microscopy research and technique May, 2007 | Pubmed ID: 17366615
Fluorescence-lifetime imaging of DNA-dye interactions within continuous-flow microfluidic systems.
Angewandte Chemie (International ed. in English) , 2007 | Pubmed ID: 17436333
Optical techniques for imaging membrane lipid microdomains in living cells.
Seminars in cell & developmental biology Oct, 2007 | Pubmed ID: 17728161
Fluorescence lifetime imaging by using time-gated data acquisition.
Applied optics Oct, 2007 | Pubmed ID: 17952172
Excitation-resolved hyperspectral fluorescence lifetime imaging using a UV-extended supercontinuum source.
Optics letters Dec, 2007 | Pubmed ID: 18059949
Stimulated emission depletion microscopy with a supercontinuum source and fluorescence lifetime imaging.
Optics letters Jan, 2008 | Pubmed ID: 18197209
Improved sectioning in a slit scanning confocal microscope.
Optics letters Aug, 2008 | Pubmed ID: 18709096
Three-dimensional molecular mapping in a microfluidic mixing device using fluorescence lifetime imaging.
Optics letters Aug, 2008 | Pubmed ID: 18709122
High-speed high-resolution imaging of intercellular immune synapses using optical tweezers.
Biophysical journal Nov, 2008 | Pubmed ID: 18723590
Multiplexed FRET to image multiple signaling events in live cells.
Biophysical journal Nov, 2008 | Pubmed ID: 18757561
Continuous-flow polymerase chain reaction of single-copy DNA in microfluidic microdroplets.
Analytical chemistry Jan, 2009 | Pubmed ID: 19055421
Live cell linear dichroism imaging reveals extensive membrane ruffling within the docking structure of natural killer cell immune synapses.
Biophysical journal Jan, 2009 | Pubmed ID: 19167281
Fluorescence lifetime optical projection tomography.
Journal of biophotonics Oct, 2008 | Pubmed ID: 19343662
A compact, multidimensional spectrofluorometer exploiting supercontinuum generation.
Journal of biophotonics Dec, 2008 | Pubmed ID: 19343675
High speed unsupervised fluorescence lifetime imaging confocal multiwell plate reader for high content analysis.
Journal of biophotonics Dec, 2008 | Pubmed ID: 19343677
Three-dimensional imaging of Förster resonance energy transfer in heterogeneous turbid media by tomographic fluorescent lifetime imaging.
Optics letters Sep, 2009 | Pubmed ID: 19756100
A fluorescence lifetime imaging scanning confocal endomicroscope.
Journal of biophotonics Jan, 2010 | Pubmed ID: 19787682
Dynamics of subsynaptic vesicles and surface microclusters at the immunological synapse.
Science signaling , 2010 | Pubmed ID: 20460647
High plasma membrane lipid order imaged at the immunological synapse periphery in live T cells.
Molecular membrane biology Aug, 2010 | Pubmed ID: 20540668
Differential modes of DNA binding by mismatch uracil DNA glycosylase from Escherichia coli: implications for abasic lesion processing and enzyme communication in the base excision repair pathway.
Nucleic acids research Apr, 2011 | Pubmed ID: 21112870
Membrane environment exerts an important influence on rac-mediated activation of phospholipase Cγ2.
Molecular and cellular biology Mar, 2011 | Pubmed ID: 21245382
Wide-field fluorescence lifetime imaging of cancer.
Biomedical optics express Aug, 2010 | Pubmed ID: 21258496
FLIM FRET technology for drug discovery: automated multiwell-plate high-content analysis, multiplexed readouts and application in situ.
Chemphyschem : a European journal of chemical physics and physical chemistry Feb, 2011 | Pubmed ID: 21337485
Adaptive phase compensation for ultracompact laser scanning endomicroscopy.
Optics letters May, 2011 | Pubmed ID: 21540976
In vivo fluorescence lifetime optical projection tomography.
Biomedical optics express Apr, 2011 | Pubmed ID: 21559145
In vivo fluorescence lifetime tomography of a FRET probe expressed in mouse.
Biomedical optics express Jul, 2011 | Pubmed ID: 21750768
Remodelling of cortical actin where lytic granules dock at natural killer cell immune synapses revealed by super-resolution microscopy.
PLoS biology Sep, 2011 | Pubmed ID: 21931537
Application of ultrafast gold luminescence to measuring the instrument response function for multispectral multiphoton fluorescence lifetime imaging.
Optics express Jul, 2011 | Pubmed ID: 21934746
Quantification of cellular autofluorescence of human skin using multiphoton tomography and fluorescence lifetime imaging in two spectral detection channels.
Biomedical optics express Dec, 2011 | Pubmed ID: 22162820
Förster resonance energy transfer imaging in vivo with approximated radiative transfer equation.
Applied optics Dec, 2011 | Pubmed ID: 22193187
Multiphoton laser microscopy and fluorescence lifetime imaging for the evaluation of the skin.
Dermatology research and practice , 2012 | Pubmed ID: 22203841
In vivo measurements of diffuse reflectance and time-resolved autofluorescence emission spectra of basal cell carcinomas.
Journal of biophotonics Mar, 2012 | Pubmed ID: 22308093
Incorporation of an experimentally determined MTF for spatial frequency filtering and deconvolution during optical projection tomography reconstruction.
Optics express Mar, 2012 | Pubmed ID: 22453413
Multiphoton multispectral fluorescence lifetime tomography for the evaluation of basal cell carcinomas.
PloS one , 2012 | Pubmed ID: 22984428
Activity of PLCε contributes to chemotaxis of fibroblasts towards PDGF.
Journal of cell science Dec, 2012 | Pubmed ID: 22992460
Fluorescence lifetime readouts of Troponin-C-based calcium FRET sensors: a quantitative comparison of CFP and mTFP1 as donor fluorophores.
PloS one , 2012 | Pubmed ID: 23152874
Automated fluorescence lifetime imaging plate reader and its application to Förster resonant energy transfer readout of Gag protein aggregation.
Journal of biophotonics May, 2013 | Pubmed ID: 23184449
Simultaneous angular multiplexing optical projection tomography at shifted focal planes.
Optics letters Mar, 2013 | Pubmed ID: 23503237
3-D stimulated emission depletion microscopy with programmable aberration correction.
Journal of biophotonics Jan, 2014 | Pubmed ID: 23788459
Multiphoton laser tomography and fluorescence lifetime imaging of melanoma: morphologic features and quantitative data for sensitive and specific non-invasive diagnostics.
PloS one , 2013 | Pubmed ID: 23923016
Rapid global fitting of large fluorescence lifetime imaging microscopy datasets.
PloS one , 2013 | Pubmed ID: 23940626
Fluorescence lifetime spectroscopy of tissue autofluorescence in normal and diseased colon measured ex vivo using a fiber-optic probe.
Biomedical optics express Feb, 2014 | Pubmed ID: 24575345
Introduction: feature issue on optical molecular probes, imaging, and drug delivery.
Biomedical optics express Feb, 2014 | Pubmed ID: 24575356
Analysis of DNA binding and nucleotide flipping kinetics using two-color two-photon fluorescence lifetime imaging microscopy.
Analytical chemistry Nov, 2014 | Pubmed ID: 25303623
Remote focal scanning optical projection tomography with an electrically tunable lens.
Biomedical optics express Oct, 2014 | Pubmed ID: 25360356
Application of time-resolved autofluorescence to label-free in vivo optical mapping of changes in tissue matrix and metabolism associated with myocardial infarction and heart failure.
Biomedical optics express Feb, 2015 | Pubmed ID: 25780727
Mesoscopic in vivo 3-D tracking of sparse cell populations using angular multiplexed optical projection tomography.
Biomedical optics express Apr, 2015 | Pubmed ID: 25909009
Correction Approach for Delta Function Convolution Model Fitting of Fluorescence Decay Data in the Case of a Monoexponential Reference Fluorophore.
Journal of fluorescence Sep, 2015 | Pubmed ID: 26063535
Homo-FRET Based Biosensors and Their Application to Multiplexed Imaging of Signalling Events in Live Cells.
International journal of molecular sciences Jun, 2015 | Pubmed ID: 26133241
Accelerated Optical Projection Tomography Applied to In Vivo Imaging of Zebrafish.
PloS one , 2015 | Pubmed ID: 26308086
Time-resolved FRET reports FGFR1 dimerization and formation of a complex with its effector PLCγ1.
Advances in biological regulation Jan, 2016 | Pubmed ID: 26482290
Visualising apoptosis in live zebrafish using fluorescence lifetime imaging with optical projection tomography to map FRET biosensor activity in space and time.
Journal of biophotonics Apr, 2016 | Pubmed ID: 26753623
Tunable fibre-coupled multiphoton microscopy with a negative curvature fibre.
Journal of biophotonics Jul, 2016 | Pubmed ID: 26989868
Quantitative in vivo optical tomography of cancer progression & vasculature development in adult zebrafish.
Oncotarget Jul, 2016 | Pubmed ID: 27259259
Screening for protein-protein interactions using Förster resonance energy transfer (FRET) and fluorescence lifetime imaging microscopy (FLIM).
Scientific reports Jun, 2016 | Pubmed ID: 27339025
Imaging of Metabolic Status in 3D Cultures with an Improved AMPK FRET Biosensor for FLIM.
Sensors (Basel, Switzerland) Aug, 2016 | Pubmed ID: 27548185
easySTORM: a robust, lower-cost approach to localisation and TIRF microscopy.
Journal of biophotonics Sep, 2016 | Pubmed ID: 27592533
Corrigendum: Screening for protein-protein interactions using Förster resonance energy transfer (FRET) and fluorescence lifetime imaging microscopy (FLIM).
Scientific reports Sep, 2016 | Pubmed ID: 27654516
Adaptive multiphoton endomicroscopy through a dynamically deformed multicore optical fiber using proximal detection.
Optics express Sep, 2016 | Pubmed ID: 27661887
Plasmonic Nanoprobes for Stimulated Emission Depletion Nanoscopy.
ACS nano Nov, 2016 | Pubmed ID: 27794591
Conformational transition of FGFR kinase activation revealed by site-specific unnatural amino acid reporter and single molecule FRET.
Scientific reports Jan, 2017 | Pubmed ID: 28045057
Frederik Görlitz*,1,
Douglas J. Kelly*,1,
Sean C. Warren1,
Dominic Alibhai2,
Lucien West3,
Sunil Kumar1,
Yuriy Alexandrov1,
Ian Munro1,
Edwin Garcia1,
James McGinty1,
Clifford Talbot1,
Remigiusz A. Serwa4,
Emmanuelle Thinon4,
Vincenzo da Paola3,
Edward J. Murray5,
Frank Stuhmeier6,
Mark A. A. Neil1,
Edward W. Tate4,
Christopher Dunsby1,7,
Paul M. W. French1
1Photonics Group, Department of Physics, Imperial College London,
2Institute for Chemical Biology, Department of Chemistry, Imperial College London,
3MRC Clinical Sciences Centre, Hammersmith Hospital,
4Chemical Biology Section, Department of Chemistry, Imperial College London,
5, Retroscreen Virology Ltd,
6Pfizer Global Research and Development, Pfizer Limited, Sandwich, Kent, UK,
7Centre for Histopathology, Imperial College London