The <em>Ex Vivo</em> Colon Organ Culture and Its Use in Antimicrobial Host Defense Studies

The intestine displays an architecture of repetitive crypt structures consisting of different types of epithelial cells, lamina propia containing immune ...

UT Southwestern Medical Center

Rotavirus-specific subclass antibody responses and cytokines, tumour necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma), interleukin-8 (IL-8), and IL-10, were measured in children 7-24 months of age with rotavirus diarrhoea (n = 29); the responses were compared with children with watery diarrhoea from whom no enteric pathogens were isolated (controls; n = 11). All children had diarrhoea for < 5 days and were enrolled from the Dhaka Hospital of the Centre for Health and Population Research. Samples of blood and stools were collected on the day of enrollment and 18-21 days after the onset of diarrhoea. Children showing a > or = 4-fold rise in antibody titre between the acute and convalescent stages were considered to have a response. The numbers of children with rotavirus-specific IgA and IgA1 responses in stool were similar in the two groups of children. In the plasma, more children with rotavirus diarrhoea had rotavirus-specific IgA, IgA1, IgG, IgG1, and IgG3 responses than did control children (P = 0.049, 0.007, 0.001, 0.002, and 0.012, respectively). IgA2 was not detectable. Among cytokines measured in supernatants from peripheral blood mononuclear cells (PBMCs) cultured for 6 and 24 hr, IFN-gamma was the only cytokine that was higher in children with rotavirus diarrhoea compared with controls (P = 0.013). Severity of illness did not correlate with nutritional status or antibody titres, but severity did correlate with TNF-alpha during the acute stage of illness. IFN-gamma correlated positively with IgG1 titres. These findings suggest a role for IFN-gamma in the pathogenesis of rotavirus infection, but this needs confirmation by other studies. The immune responses described are relevant to future vaccine trials, as immune responses in vaccinees should mimic those in natural infection.

Rotavirus-specific subclass antibody and cytokine responses in Bangladeshi children with rotavirus diarrhoea.

Hepatitis B virus (HBV) infection is one of the most prevalent public health problems worldwide, and causes 1 million deaths annually. In Bangladesh, information about prevalence of HBV infection is scarce, and there is no available data on HDV infection. We determined rates of HBsAg and anti-HBc seropositivity in asymptomatic, healthy children (n = 181) and adults (n = 354) presenting to referral facilities in Dhaka, Bangladesh, and tested a separate group of HBsAg-positive patients (n = 180) for prevalence of HDV. Testing of serum was also performed for signs of liver disease. Overall, seropositivity of HBsAg and anti-HBc in studied subjects was 3 per cent (16/534) and 21.1 per cent (113/534), respectively. Prevalence of HBsAg was highest in the 5- to 9-year-old (8.5 per cent, 7/82) and 10- to 14-year-old (5.9 per cent, 2/34) age groups. Unlike HBsAg, prevalence of anti-HBc was lower in children (14.9 per cent in those below the age of 15) than adults (24.4 per cent in those aged 20-34 years) (p < 0.05). Most HBsAg-positive individuals were symptomatic (n = 125, 69.4 per cent). A high rate (24.4 per cent, 44/180) of simultaneous infection with HDV was observed among HBsAg-positive subjects, with higher rates in older individuals. Anti-HDV seropositivity rate was similar among asymptomatic (21.8 per cent, 12/55) and symptomatic (25.6 per cent, 32/125) HBsAg carriers. Our data suggest that Bangladesh is of moderate endemicity for HBV infection, and has relatively high rates of co-infection with HDV. Control HBV and HDV infection in Bangladesh may be best achieved by targeting preschool children, which could fit readily within the existing EPI schedule.

Seroepidemiology of hepatitis B and delta virus infections in Bangladesh.

In view of considerable differences of opinion regarding the reproductive steroid hormonal pathogenesis in catamenial epilepsy, hormonal analysis of estrogen and progesterone in catamenial epileptics for a precise correlation is of significant importance.

Influence of steroid hormones in women with mild catamenial epilepsy.

To describe clinical features and seizure dispersion in precatamenial/pericatamenial epilepsy patients.

Clinical and neurological study of women with precatamenial epilepsy.

Stroke is one of the major causes of morbidity and mortality throughout the world. Reports about the role of Chlamydia pneumoniae infection in the development of atherosclerosis have been reported in many studies. The aim of the study was to evaluate the association between Chlamydia pneumoniae infection and ischemic stroke.

Association of Chlamydia pneumoniae serology and ischemic stroke.

To study the clinical and radiological characteristics of multiple sclerosis (MS) with onset below 18 years.

Clinical and radiological study of Iraqi multiple sclerosis patients with childhood onset.

To assess the prognostic factors of relapsing remitting multiple sclerosis (RR-MS) in Iraqi patients.

Disability and prognosis of relapsing remitting multiple sclerosis, is it different in Iraqi patients?

Objective. To assess differences in age of onset, hypertension duration, type of drug, treatment compliance, and salt-free diet compliance between patients with stroke and myocardial infarction. Patients and Methods. The study was conducted in 3 hospitals in Baghdad between June 2010 and June 2011. First group includes 81 stroke patients (36 females and 45 males), age ranges between (33-82 years). Second group includes 110 myocardial infarction patients (46 females and 64 males), ages ranges from (23-76 years). Results. Salt-free diet noncompliance was seen in 69% and 62% of Myocardial infarction and stroke groups, respectively. Silent hypertension was seen in 6.3% and 19.7% of myocardial infarction and stroke groups, respectively. Noncompliant on antihypertensive therapy was seen in 61%, 71%, and 48% of the total, myocardial infarction, and stroke groups, respectively. The drug type was 24% angiotensin converting enzyme inhibitor, 18.8% combined drugs, 16.2% Beta Blocker, 11% angiotensin 11 receptor blocker, 10.4% calcium channel blocker and 7.3% diuretic. In stroke group, the commonest drug was 23% angiotensin converting inhibitor and the least (5%) was angiotensin receptor blocker. In myocardial infarction group, the commonest drug was 25% Angiotensin Converting Inhibitor and the least (8%) was diuretic. Discussion and Conclusion. Silent hypertension was high in Iraq. Salt-free diet noncompliance was high in both groups; drug noncompliance was significantly higher in patients with myocardial infarction. Angiotensin 11 receptor blocker use was associated significantly with myocardial infarction more than in stroke.

Hypertension as a risk factor: is it different in ischemic stroke and acute myocardial infarction comparative cross-sectional study?

Intracranial hydatid cyst. Clinical features and outcomes of surgical treatment of a series of 8 Iraqi cases.

The epidemiology of multiple sclerosis (MS) is rapidly changing in many parts of the world. In a geographic area that was previously associated with low prevalence, recent studies showing high prevalence and fast rising incidence of MS in the Levant countries led us neurologists of this region to meet in a consensus panel, in order to share our latest findings in terms of MS epidemiology and consent on MS management in the Levant. Twelve neurologists and MS experts representing various countries of the Levant (Lebanon, Syria, Jordan and Iraq) have met in Beirut on the 17(th) of February 2013, shared their latest epidemiological findings, discussed recent MS aspects in the region, and drafted a consensus on MS management relevant to this geographic area.

Multiple sclerosis in the Levant: a regional consensus statement.

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To find an association between human leukocyte antigen (HLA) class II DRB1, DRB3, DRB4, and DRB5 alleles frequencies in a sample of Iraqi patients with Guillain-Barre syndrome (GBS) and compare with a healthy control group.

Hasan Zaki

Department of Pathology

UT Southwestern Medical Center

The Ex Vivo Colon Organ Culture and Its Use in Antimicrobial Host Defense Studies

Hasan Zaki

.css-o250i3{font-size:18px;font-family:Open Sans,sans-serif;line-height:21.6px;}Department of Pathology

UT Southwestern Medical Center

The Ex Vivo Colon Organ Culture and Its Use in Antimicrobial Host Defense Studies

Department of Pathology