daily-phototherapy-with-red-light-to-regulate-candida-albicans

Daily Phototherapy with Red Light to Regulate <em>Candida albicans</em> Biofilm Growth

Here, we present a protocol to assess the outcomes of per diem red light treatment on the growth of Candida albicans biofilm. To increase the planktonic ...

Indiana University School of Dentistry

: Infections caused by  spp. have been associated with formation of a biofilm, i.e. a complex microstructure of cells adhering to a surface and embedded within an extracellular matrix (ECM). : The ECMs of a wild-type (WT, SN425) and two  mutant strains, Δ/Δ tec1 (CJN2330) and Δ/Δ efg1 (CJN2302), were evaluated. Colony-forming units (cfu), total biomass (mg), water-soluble polysaccharides (WSPs), alkali-soluble polysaccharides (ASPs), proteins (insoluble part of biofilms and matrix proteins), and extracellular DNA (eDNA) were quantified. Variable-pressure scanning electron microscopy and confocal scanning laser microscopy were performed. The biovolume (μm/μm) and maximum thickness (μm) of the biofilms were quantified using COMSTAT2. : ASP content was highest in WT (mean ± SD: 74.5 ± 22.0 µg), followed by Δ/Δ tec1 (44.0 ± 24.1 µg) and Δ/Δ efg1 (14.7 ± 5.0 µg). The protein correlated with ASPs ( = 0.666) and with matrix proteins ( = 0.670) in the WT strain. The population in Δ/Δ efg1 correlated with the protein ( = 0.734) and its biofilms exhibited the lowest biomass and biovolume, and maximum thickness. In Δ/Δ tec1, ASP correlated with eDNA ( = 0.678). : ASP production may be linked to  cell filamentous morphology.

Inactivation of genes TEC1 and EFG1 in  influences extracellular matrix composition and biofilm morphology.

Phototherapy has been proposed as a direct means of affecting local bacterial infections. However, the use of phototherapy to prevent fungal biofilm development has received comparatively less attention. This study aimed to determine the effects of red light treatment and blue light treatment, without a photosensitizer, on the development of Candida albicans biofilm. During the development of 48-h biofilms of C. albicans SN 425 (n = 10), the biofilms were exposed twice-daily to noncoherent blue and red light (LumaCare; 420 nm and 635 nm). The energy density applied was 72 J cm for blue light and 43.8 J cm, 87.6 J cm, and 175.5 J cm for red light. Positive control (PC) and negative control (NC) groups were treated twice-daily for 1 min with 0.12% chlorhexidine (CHX) and 0.89% NaCl respectively. Biofilms were analyzed for colony forming units (CFU), dry-weight, and exopolysaccharides (EPS-soluble and EPS-insoluble). Data was analyzed by one-way ANOVA and Tukey post hoc test (α = 0.05). Dry-weight was lower than NC (p < 0.001) and approached PC levels with both red and blue light treatments. CFU were also lower in groups exposed to blue light and higher durations of red light (p < 0.05). EPS-soluble and EPS-insoluble measures were variably reduced by these light exposures. In conclusion, twice-daily exposure to both blue and red lights affect the biofilm development and physiology of polysaccharide production and are potential mechanisms for the control of C. albicans biofilm matrix development.

Twice-daily red and blue light treatment for Candida albicans biofilm matrix development control.

Evaluate the effect of low-temperature plasma (LTP) on an anaerobic biofilm and on the biological response of an in vitro reconstituted gingival epithelium tissue.

Simone Duarte

Department of Cariology,
Operative Dentistry and Dental Public Health,
Department of Cariology, Operative Dentistry and Dental Public Health

Indiana University School of Dentistry

Daily Phototherapy with Red Light to Regulate Candida albicans Biofilm Growth

Simone Duarte

Department of Cariology,Operative Dentistry and Dental Public Health,Department of Cariology, Operative Dentistry and Dental Public Health

Indiana University School of Dentistry

Daily Phototherapy with Red Light to Regulate Candida albicans Biofilm Growth

Department of Cariology,
Operative Dentistry and Dental Public Health,
Department of Cariology, Operative Dentistry and Dental Public Health