Production of Human Neurogenin 2-Inducible Neurons in a Three-Dimensional Suspension BioreactorJeanette Wihan 1, Isabell Karnatz 1, Isabelle Sébastien 1, Ralf Kettenhofen 1, Benjamin Schmid 2, Christian Clausen 2, Benjamin Fischer 1, Rachel Steeg 3, Heiko Zimmermann 1,4,5,6, Julia C. Neubauer 1,4
1Fraunhofer Project Center for Stem Cell Process Engineering, Fraunhofer Institute for Biomedical Engineering IBMT, 2Bioneer A/S, 3Fraunhofer UK Research Ltd, Technology and Innovation Centre, 4Fraunhofer Institute for Biomedical Engineering IBMT, 5Department of Molecular and Cellular Biotechnology, Saarland University, 6Facultad de Ciencias del Mar, Universidad Católica del Norte
This article describes a protocol for the generation of human induced pluripotent stem cell-derived neurons in a benchtop 3D suspension bioreactor.
