Quantification and Whole Genome Characterization of SARS-CoV-2 RNA in Wastewater and Air SamplesJosé Gonçalves 1,2,3, Priscilla Gomes da Silva 4,5,6, Tom Koritnik 3, Martin Bosilj 3, Andrés Torres-Franco 1,2, Israel Diaz 1,2, Elisa Rodriguéz 1,2, Eva Marcos 2,3, João R. Mesquita 4,5, Pedro García-Encina 1,2
1Institute of Sustainable Processes, University of Valladolid, 2Department of Chemical Engineering and Environmental Technology, University of Valladolid, 3Department of Public Health Microbiology, National Laboratory of Health, Environment and Food, 4ICBAS - School of Medicine and Biomedical Sciences, Porto University, 5Epidemiology Research Unit (EPIUnit), Instituto de Saúde Pública da Universidade do Porto, 6Laboratório para a Investigação Integrativa e Translacional em Saúde Populacional (ITR)
This protocol aims to quantify SARS-CoV-2 RNA in wastewater and air samples to be used for wastewater-based epidemiology studies and to assess the exposure risk to SARS-CoV-2 in indoor and outdoor aerosols. This protocol also describes a tiled amplicon long-template sequencing approach for SARS-CoV-2 whole genome characterization.
