Maintaining and Assessing Various Tissue and Cell Types of the Eye Using a Novel Pumpless Fluidics SystemMatthew K. Grumbine 1, Varun Kamat 2, Khang Bao 1, Trevor Crupi 1, Kedar Mokate 2, Rayne Lim 3, Jennifer R. Chao 3, Brian M. Robbings 4, Daniel T. Hass 4, James B. Hurley 4, Ian R. Sweet 1,2
1EnTox Sciences, Inc, 2UW Medicine Diabetes Institute, University of Washington, 3Department of Ophthalmology, University of Washington, 4Department of Biochemistry, University of Washington
Real-time analysis of live tissue yields important functional and mechanistic data. This paper describes the protocols and critical variables to ensure accurate and reproducible generation of data by a novel and pump-free multi-channel fluidics system that maintains and assesses a wide range of tissue and cell models.
