Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine

3 ARTICLES PUBLISHED IN JoVE

Neuroscience

Application of Flow Cytometric Analysis for Measuring Multiple Mitochondrial Parameters in 3D Brain Organoids

Sharika Marjan^1,2, Tsering Yangzom^1,2, Cecilie Katrin Kristiansen^1,3, Anbin Chen⁴, Laurence A. Bindoff^1,5,6, Kristina Xiao Liang^1,3

¹Department of Clinical Medicine (K1), University of Bergen, ²Centre for International Health, Department of Global Public Health and Primary Care, University of Bergen, ³Neuro-SysMed, Center of Excellence for Clinical Research in Neurological Diseases, Haukeland University Hospital, ⁴Department of Neurosurgery, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, ⁵Department of Neurology, Haukeland University Hospital, ⁶National Advisory Unit for Congenital Metabolic Diseases, Oslo University Hospital

This protocol reports a unique method of using a streaming cytometer and multiple antibodies for simultaneous assessment of multiple mitochondrial functional parameters, including changes in mitochondrial volume, amounts of the mitochondrial respiratory chain (MRC) complex subunits, and mitochondrial DNA (mtDNA) replication.

Biology

Establishment of Facial Nerve Injury Rat Model for Idiopathic Facial Paralysis Research

Xie Yandong^1,2, Ying Tingting^1,2, Liu Liang³, Liu Mingxi³, Wang Ran³, Li Shiting^1,2, Xin Zhang^1,2

¹Department of Neurosurgery, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, ²The Cranial Nerve Disease Center, Shanghai Jiaotong University, ³Department of Neurosurgery, Affiliated Nanjing Brain Hospital, Nanjing Medical University

The present protocol establishes a facial nerve injury rat model using microscopy to investigate the diagnostic and therapeutic mechanisms of idiopathic facial paralysis.

Developmental Biology

Generation of 3D Midbrain Organoids from Human-Induced Pluripotent Stem Cells

Tsering Yangzom^1,2, Anbin Chen^1,3, Kristina Xiao Liang¹

¹Department of Clinical Medicine (K1), University of Bergen, ²Centre for International Health, Department of Global Public Health and Primary Care, University of Bergen, ³Department of Neurosurgery, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine

This protocol describes a novel method for creating 3D midbrain organoids from human induced pluripotent stem cells, guiding their formation to mimic native midbrain tissue, thereby aiding in the study of development and disorders.