Quantification, Viability Assessment, and Visualization Strategies for Acinetobacter BiofilmsJoo-Sung Kim 1,2, Jihoon Lim 3
1Korea Food Research Institute, 2Department of Food Biotechnology, Korea University of Science and Technology, 3Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute
This protocol describes the preparation of the inoculum, the biofilm quantification on microtiter plates using crystal violet dye, the viable count in biofilms, and the visualization of biofilms of Acinetobacter.