Primordial Germ Cell Cryopreservation and Revival of Drosophila StrainsKaori Nishimura *1, Miho Asaoka *2, Yurina Sakamaki *2, Tatsuya Fukumoto *3,4, Daisuke Tanaka 3, Satoru Kobayashi 2, Toshiyuki Takano-Shimizu-Kouno 1
1KYOTO Drosophila Stock Center, Kyoto Institute of Technology, 2Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, 3Research Center of Genetic Resources, National Agriculture and Food Research Organization, 4Shizuoka Prefectural Ogasa High School
A long-term preservation method for Drosophila strains as an alternative to the frequent transfer of adult flies to fresh food vials is highly desirable. This protocol describes the cryopreservation of Drosophila primordial germ cells and strain revival via their transplantation to agametic host embryos.
