Profiling of Surface Protein Epitopes on Viral Particles by Multiplex Dual-Reporter StrategyMaryam Sahi 1, Sarah Andersson 1, Cecilia Mattson 1, Matilda Dale 1, Sofia Kagiolglou 1, Camilla Hofström 2, Helena Persson 2, Jonas Klingström 3,4, Francesca Chiodi 5, Claudia Fredolini 1
1Affinity Proteomics-Stockholm Unit, SciLifeLab, Division of Affinity Proteomics, Department of Protein Science, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), KTH Royal Institute of Technology, 2Human Antibody Therapeutics Unit, SciLifeLab, Division of Drug Discovery and Development, Department of Protein Science, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), KTH Royal Institute of Technology, 3Center for Infectious Medicine, Department of Medicine Huddinge, Karolinska Institutet, 4Public Health Agency of Sweden, 5Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet
Here, we describe a newly developed multiplex fluorescent immunoassay that uses a dual-reporter flow cytometric system to concurrently detect two unique spike protein epitopes on intact severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral particles that had been captured by angiotensin-converting enzyme-2-coupled magnetic microspheres.
