Scoring Central Nervous System Inflammation, Demyelination, and Axon Injury in Experimental Autoimmune EncephalomyelitisCarmen C. Ucciferri 1, Annette Gower 2, Nuria Alvarez-Sanchez 1,2, Heather Whetstone 3, Valeria Ramaglia 1, Jennifer L. Gommerman 1, Koroboshka Brand-Arzamendi 2, Raphael Schneider 2,4, Shannon E Dunn 1,2,5
1Department of Immunology, University of Toronto, 2Keenan Research Centre for Biomedical Science of St. Michael’s Hospital, 3Sickkids Research Institute, The Hospital for Sick Children, 4BARLO MS Centre, St. Michael’s Hospital, 5Women’s College Research Institute, Women’s College Hospital
Experimental autoimmune encephalomyelitis (EAE) serves as an animal model of multiple sclerosis. This article describes an approach for scoring spinal cord inflammation, demyelination, and axonal injury in EAE. Additionally, a method to quantify soluble neurofilament light levels in the mice serum is presented, facilitating the assessment of axonal injury in live mice.
