Isolation and Culture of Primary Human Mammary Epithelial CellsLinfeng Qian *1, Jiaxi Yan *1, Shiqi Chen 1, Maryam Mohanmmed Abbas Karekad 2, Yue Wu 3,4, Xunwei Wu 5, Xiaohong Xu 3,4, Xiaoqun Zhang 6,7
1The First Clinical Medical College of Zhejiang Chinese Medical University, 2The International Education College of Zhejiang Chinese Medical University, 3Department of Breast Surgery, The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Traditional Chinese Medicine), 4Bozhou District Hospital of Traditional Chinese Medicine, 5Engineering Laboratory for Biomaterials and Tissue Regeneration, Ningbo Stomatology Hospital, Ningbo, China and Savaid Stomatology School, Hangzhou Medical College, 6Department of Surgery, Kaihua District, The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Traditional Chinese Medicine), 7Kaihua County Hospital of Traditional Chinese Medicine
The present study reports an easier, time-saving, and economical protocol to efficiently isolate and grow primary human mammary epithelial cells (HMECs) from small amounts of mammary tissue. This protocol is suitable for quickly producing primary HMECs both for laboratory and clinical applications.
