Controlled Reversible Visceral Arterial Ischemia, Venous Congestion and Combined Malperfusion via Midline Laparotomy in RatsAlexander Studier-Fischer 1,2,3,4, Berkin Özdemir 1,3,4, Karl-Friedrich Kowalewski 2,3,4, Caelan Max Haney 2,3,4,5, Luisa Egen 2,3,4, Maurice Stephan Michel 2,3,4, Markus Mieth 1, Zoltan Czigany 1, Gabriel Alexander Salg 1, Henrik Nienhüser 1, Franck Billmann 1
1Department of General, Visceral and Transplantation Surgery, Heidelberg University Hospital, 2Department of Urology and Urosurgery, University Medical Center Mannheim, Medical Faculty of the University of Heidelberg, 3Division of Intelligent Systems and Robotics in Urology (ISRU), German Cancer Research Center (DKFZ) Heidelberg, 4DKFZ Hector Cancer Institute at the University Medical Center Mannheim, 5Department of Urology, University Hospital Leipzig
This article introduces a standardized procedure for controlled, reversible malperfusion of visceral organs in rat models. The aim is to induce these malperfusion states with a high degree of methodological certainty and control while maintaining technical simplicity and error resilience.
