Standardization of a Novel Semi-Automatic Software for Neurite Outgrowth Measurement Giada Musso *1, Sofia Dotta *1,2, Amisha Parmar 1,2, Daniela Maria Rasà 1,2,3, Ferdinando Di Cunto 1,2, Letizia Marvaldi 1,2
1Neuroscience Institute Cavalieri Ottolenghi, University of Turin, 2Department of Neuroscience Rita Levi-Montalcini, University of Turin, 3University School for Advanced Studies IUSS Pavia
Neurite outgrowth assays provide a quantitative value about regenerative neuronal processes. The advantage of this semi-automatic software is that it segments cell bodies and neurites separately by creating a mask and measures various parameters such as neurite length, number of branch points, cell-body cluster area, and number of cell clusters.
