Accedi

DeSales University

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

The Use of the Ex Vivo Chandler Loop Apparatus to Assess the Biocompatibility of Modified Polymeric Blood Conduits

Joshua B. Slee^1,2, Ivan S. Alferiev^1,2, Robert J. Levy^1,2, Stanley J. Stachelek^1,2

¹Division of Cardiology, Department of Pediatrics, The Children's Hospital of Philadelphia, ²University of Pennsylvania Perelman School of Medicine

Blood exposure to polymeric blood conduits initiates the foreign body reaction that has been implicated in clinical complications. Here, the Chandler Loop Apparatus, an experimental tool mimicking blood perfusion through these conduits, is described. Appendage of recombinant CD47 results in decreased evidence of the foreign body reaction on these conduits.

Genetics

Using a GFP-tagged TMEM184A Construct for Confirmation of Heparin Receptor Identity

Sara Lynn N. Farwell¹, Joshua B. Slee², Yaqiu Li¹, Linda J. Lowe-Krentz¹

¹Department of Biological Sciences, Lehigh University, ²Department of Natural Science, DeSales University

A construct encoding TMEM184A with a GFP tag at the carboxy-terminus designed for eukaryotic expression, was employed in assays designed to confirm the identification of TMEM184A as a heparin receptor in vascular cells.