Trophoblast Cell Recovery from Angiogenesis-Tube Formation Assay for Differentiation Marker Expression AnalysisRodrigo Escalona-Rivano 1, Delia I. Chiarello 1, Lorena Carvajal 1, Ivo Carrasco-Wong 1, Jaime A. Gutiérrez 1
1Facultad de Medicina y Ciencia, Universidad San Sebastián, Santiago, Chile
The present protocol allows the recovery of tube-like structures formed in the classical in vitro angiogenesis-tube formation assay where cells, such as trophoblast cells, organize in capillary-like structures over a layer of extracellular basement membrane matrix. The recovered structures are used for RNA and protein isolation for various biochemical analyses.