Glasgow Biofilm Research Network

1 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Organotypic Tissue Model Systems for Investigating Host-Pathogen Interactions In Vitro
Muhanna Alshehri 1,2, Saeed Alqahtani 1,2,3, Pranitha Murali 2,4,5, Christopher Delaney 1,2, William Johnston 2,4,5, Jason L. Brown 1,2
1Oral Sciences Research Group, Glasgow Dental School, School of Medicine, College of Medical, Veterinary and Life Sciences, University of Glasgow, 2Glasgow Biofilm Research Network, 3Department of Restorative Dental Sciences, College of Dentistry, Jouf University, 4Department of Biological and Biomedical Sciences, Glasgow Caledonian University, 5Safeguarding Health through Infection Prevention Research Group, Glasgow Caledonian University

The present protocol outlines the application of organotypic tissue models for investigating host-pathogen interactions in vitro. Specifically, the model system described uses a multi-layered oral epithelium exposed to microorganisms associated with biofilms (dental plaque) within the context of oral health versus disease.

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