Accedi

Braunschweig University of Technology

3 ARTICLES PUBLISHED IN JoVE

Neuroscience

Targeting Olfactory Bulb Neurons Using Combined In Vivo Electroporation and Gal4-Based Enhancer Trap Zebrafish Lines

Kenric J. Hoegler¹, Martin Distel², Reinhard W. Köster³, John H. Horne¹

¹Department of Biology, Pace University, ²Cellular and Molecular Medicine, University of California, San Diego, ³Division of Cell Biology and Cell Physiology, Zoological Institute, Braunschweig University of Technology

The temporal and spatial resolution of genetic manipulations determines the spectrum of biological phenomena that they can perturb. Here we use temporally and spatially discrete in vivo electroporation, combined with transgenic lines of zebrafish, to induce expression of a GFP transgene specifically in neurons of the developing olfactory bulb.

Developmental Biology

Culture and Transfection of Zebrafish Primary Cells

Giulio Russo^1,2, Franziska Lehne¹, Sol M. Pose Méndez¹, Stefan Dübel², Reinhard W. Köster¹, Wiebke A. Sassen¹

¹Division of Cellular and Molecular Neurobiology, Zoological Institute, Braunschweig University of Technology, ²Department of Biotechnology, Institute of Biochemistry, Biotechnology and Bioinformatics, Braunschweig University of Technology

We present an efficient and easy-to-use protocol for preparing primary cell cultures of zebrafish embryos for transfection and live cell imaging as well as a protocol to prepare primary cells from adult zebrafish brain.

Neuroscience

In vivo Imaging of Fully Active Brain Tissue in Awake Zebrafish Larvae and Juveniles by Skull and Skin Removal

Paul Schramm¹, Florian Hetsch², Jochen C. Meier³, Reinhard W. Köster¹

¹Cellular and Molecular Neurobiology, Zoological Institute, Technische Universität Braunschweig, ²Institute of Pathophysiology, University Medical Center of the Johannes Gutenberg University, ³Cell Physiology, Zoological institute, Technische Universität Braunschweig

Here we present a method to image the zebrafish embryonic brain in vivo upto larval and juvenile stages. This microinvasive procedure, adapted from electrophysiological approaches, provides access to cellular and subcellular details of mature neuron and can be combined with optogenetics and neuropharmacological studies for characterizing brain function and drug intervention.